Small Rna Library Prep Kit

This version of the kit is intended for use with your barcode system and please note that index primers for multiplexing are not included in this kit.

Small rna library prep kit. Truseq small rna library prep kit reference guide 15004197 instructions for preparing libraries using the truseq small rna library prep kit. Nextflex small rna seq kit v3 a small rna library prep kit for illumina sequencing which provides randomized adapters for bias reduction along with gel free or low input protocols. Whether you are generating libraries for small rna from plasma urine or other challenging sample types norgen s patented resin technology will enhances recovery of desired library intermediates or final products. Unprecedented reduction of adapter dimer formation allows completely gel free small rna library prep when starting with 200 ng of total rna.

It includes all the necessary reagents for the complete workflow including barcodes for multiplexing. The novel workflow has been optimized to minimize adaptor dimers while producing high yield high diversity libraries. The unique workflow of the nebnext small rna library prep kits addresses the challenge of minimization of adaptor dimers while achieving production of high yield diverse multiplex libraries in a simple protocol. Multiplexing of up to 96 samples multiplexing of up to 96 samples is possible with complimentary i7 indexes provided in the kit.

The small rna seq library prep kit provides a protocol for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. The small rna seq library prep kit provides reagents for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. Zymo seq ribofree total rna seq library kit is the fastest easiest rna seq library prep kit available to make stranded total rna libraries. Norgen offers optimized library preparation kits that accommodate a wide range of sample types and input quantities.

The automation friendly protocol minimizes hands on time to generate sequencer ready stranded indexed libraries depleted of rrna and globin in as little as 3 5 hours. The nebnext multiplex small rna library prep kit for illumina index primers 1 48 contains the adaptors primers enzymes and buffers required to convert small rnas into indexed libraries for next generation sequencing on the illumina platform. The total yield was 0 91 gb with 97 of bases at or above q30. These libraries were sequenced on the miniseq system using the miniseq high output reagent kit at a 1 x 36 bp read length configuration.

This is possible thanks to the dual approach used for adapter dimer reduction. The human brain reference rna hbrr and universal human reference rna uhrr samples were prepared using the truseq small rna library prep kit.